Insertion sequence of 113 bp was detected in 5' upstream region of alpha-Amy 3 in one accession of Ae. tauschii from Iran. This insertion was considered as a Stowaway-like transposable element because of its short length, conserved terminal inverted repeats, and the presence of target site duplication (TA). Since Stowaway-like elements exist abundantly in Ae. tauschii genome, IMP (Inter-MITE Polymorphisms) analysis should be applicable to detect genetic variation. The analysis of Ae. tauschii introduced from Shaanxi Province showed no polymorphism by RAPD analysis. Then, IMP analysis was performed for 49 accessions of Ae. tauschii introduced from Shaanxi Province. Polymorphism was detected in two accessions (AT55 and AT56) from Yangling, in which a fragment of 220bp and 266bp was absent, respectively. The latter fragment was amplified in all accessions from Shaanxi Province excluding AT56, while it was amplified only in one accession (AT4, var. typica) from Afghanistan among 31 accessions from other areas. Nucleotide sequence of this fragment was almost same between AT4 and accessions from Shaanxi Province, suggesting that Ae. tauschii established in Shaanxi Province was principally derived from very limited population consisted of AT4 type.

P-13 N. Kodarma^1*, M. Ishii², K. Kato¹, K.Takeda² (¹Fac Agr, Okayama Univ, ²Res Inst Biores, Okayama Univ, *gagl4Ol8@cc.okayama-u.ac.jp)
PCR based marker for the selection of spring growth habit genes (Sgh) of barley

Most of the Japanese two-rowed barley are of spring type carrying sgh gene, and are considered to be inferior in cold tolerance and productivity. For the breeding of winter type cultivar, we tried to develop PCR marker for the selection of spring growth habit genes. The Japanese six-rowed winter barley, Hayakiso 2 and Dairokkaku 1, and their NILs for Sgh genes were used in this study. STS primers were designed to amplify 9 RFLP loci which linked to sgh or Sgh2 genes, and nucleotide sequence of PCR products was compared among the NILs. Among 9 RFLP loci analyzed, polymorphism was detected in XB-Amy1 whose primers were designed to amplify the third intron. The size of PCR products was different between Hayakiso 2 (sgh) (1200bp) and Hayakiso 2 (Sgh) (1350bp). The analysis of their F₂ population showed that recombination value between XB-Amy1 and sgh was 4.4 cM which was comparable to the map distance reported so far. Most of the Japanese two-rowed cultivars with spring growth habit proved to be of 1200bp type, while foreign cultivars with winter growth habit, such as Igri and Proctor, showed a band of 1350bp. Therefore, a PCR based marker of XB-Amyl can be utilized for the introduction of Sgh gene into the Japanese two-rowed barley.

P-14 I. Onishi, A. Hongo and H. Miura (Obihiro Univ Agric & Vet Med)
Quantitative evaluation of shattering traits in hexaploid wheat

The speltoid-suppressing gene Q on chromosome 5A of common wheat (Triticum aestivum L.) is supposed to associate with free-threshing and square-headed spikes with non-fragile rachies. On the other hand, T. spelta and T. macha carrying the q allele are characterized by a narrow and long spike with brittle rachies. Rachies brittleness affects shattering tolerance that is important for cultivated wheat species. While genetic variation in shattering traits has been suggested, quantitative evaluation of the traits has not yet been established. So we developed a system measuring kinetic energy of shattering. The system can measure physical force and moving distance with 1 kg load-cell and displacement when rachies snap. Using this system, genetic variation for shattering traits within T. aestivum and T. spelta, as well as dosage effect of the Q allele, was investigated.