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Wheat Information Service
Number 93: 32-34 (2001)
Research information


'Thatcher'-avirulent Ieaf rust pathotypes from India

A. N. Mishra1, Kamini Kaushal1, S. K Jain2 and H. N. Pandey1

1Indian Agricultural Research Institute, Regional Wheat Research Station, Indore 452 001, India
2Directorate of Wheat Research (DWR), Regional Station, Flowerdale, Shimla 171,002, India


Bread wheat cultivar Thatcher is documented to carry a single gene ( Lr22b ) for adult plant resistance to leaf rust (McIntosh et al. 1995), while its seedling is susceptible to wheat leaf rust. Therefore, it has been used as the genetic background to develop the near-isogenic lines for resistance to leaf rust. However, leaf rust pathotypes avirulent to Thatcher seedlings have been known to occur in Ethiopia and Morocco until now (Huerta-Espino and Roelfs 1992). The present communication reports three Indian leaf rust pathotypes carrying avirulence to Thatcher seedlings. These three pathotypes of leaf rust, OR8, 0R8-1, and OR9, belonging to standard leaf rust races 11, 63, and 106 are maintained at the DWR-Shimla. These pathotypes were also avirulent to seedlings of the 14 near-isogenic lines with Thatcher background carrying a resistance gene to leaf rust, Lr1, 2a, 2c, 3a, 10, 13, 14a, 15, 17, 18, 19, 23, 24 or 26. They showed virulence to a bread wheat Agra Local, to which the Ethiopian and Moroccan isolates were avirulent (Mishra 1996). These three pathotypes produced the low infection types on Thatcher seedlings with minor, but consistent differences (Table 1). These pathotypes were tested with 20 lines each of bread and durum wheats (Table 1), since the Thatcher- avirulent leaf rust isolates from Ethiopia and Morocco tended to be avirulent to bread wheats but virulent to durum wheats (Huerta-Espino and Roelfs 1992). Leaf rust pathotype 121R63-1 (77-5), carrying virulence to Thatcher and many of the known Lr genes, was also included for comparison. Seedlings of the test lines including suitable cheeks were evaluated at 18-27C (temperatures mostly ranging between 20-25C) using standard glasshouse procedures (Stakman et al. 1962). While the three Thatcher- avirulent pathotypes were generally avirulent to bread wheats, they displayed differential interaction with durum wheats (Table 1). In contrast, pathotype 121R63-1 (77-5) was virulent to all the bread wheat lines, but avirulent to most of the durum wheats (Table 1). Similar differences in the seedling response of durum wheat a and bread wheat to the leaf rust races 77 and 106 have earlier been reported from India (Pandey and Rao 1984). These findings emphasize the need for separate protocols with regard to the choice of leaf rust pathotypes for evaluating leaf rust resistance in bread and durum wheats.

One wheat cultivar Kanred, a parental line of Thatcher, was speculated as the source of seedling resistance to the Thatcher-avirulent Ethiopian leaf rust isolates based on their comparison of the infection types (Mishra and Roelfs 1995). The source of seedling resistance in Thatcher to the three Thatcher-avirulent Indian leaf rust pathotypes is not known, and further studies are being conducted to explain it.


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