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Materials and methods

Seeds of following species of Aegilops, Triticum and Hordeum were available at Atomic Energy Agricultural Research Centre (AEARC) Tandojam during 1993-94.

a) Aegilops
1. Ae. ovata L. Raspail'CUCUMOMO'
2. Ae. kotschyi Boiss. 'CUCUSVSV'
3. Ae. vavilovii (Zhuk.) Chenn. 'DDMcrMcrSPSP'
4. Ae. tauschii Coss. Schmal 'DD'

b) Triticum
5. T. monococcum L. 'AA'(small spelt)
6. T. dicoccum (Schrank) Schubl.'AABB'(Emmer)
7-10. T. dicoccoides (Korn.) Schweinf. 'AABB' (Wild emmer) (Accession Nos: 49,53,54, 60)
11. T. polonicum L.'AABB'(Polish)
12. T. turgidum L.'AABB'(Cone)
13. T. aestivum L. Thell. 'AABBDD' (Bread)
14. T. sphaerococcum Perc.'AABBBDD'(Indian dwarf or shot)
15. T. spelta L.'AABBDD'(Dinkel).

c) Hordeum
16. H. vulgare L.'HH'

Data on following parameters were collected
Grain weight: It was recorded in grams after counting 100 grains at random. Protein: Protein contents were determined by Udy dye binding capacity method (Udy 1971). Lysine: Lysine contents were determined by a modified DBC method (100 mg flour, 0.45 mg/ml dye concentration) (Khan 1978). Lysine values were expressed on flour (mg/g) and protein (%) basis.

Whole meal flour was used for analytical purpose and all the data were calculated on dry weight basis.


Results and discussion

Wide genetic variability was estimated in the wild species of Aegilops, Triticum and Hordeum for grain weight, protein and lysine content (Table 1). Wild grasses involving species ofAegilops and one accession (No.60) of T. dicoccoides possessed lower values for grain weight, but relatively higher values for protein and lysine content establishing a negative relationship. Hundred grain weight of Czechoslovak Aegilops collection ranged between 0.34 and 1.70g, Aegilops tauschii between 0.86 and 1.70g (Holubee et al. 1992). Similar trend was observed among different species of Triticum. Triticum polonicum with bold seeds possessed maximum 100-grain weight of 6.5g, which was significantly higher than other species of Triticum and Hordeum (ranging between 0.47g to 3.86g).

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