(go to
KOMUGI Home) (go
to WIS List) (go
to NO.84 Contents)
Wheat Information Service
Number 84: 25-32 (1997)
Research article
The rice Actl promoter gave high activity
of transient gusA expression in callus, immature embryos and
pollen embryoids of common wheat and its relatives following particle
bombardment
S. Takumi, M. Otani and T. Shimada
Research Institute of Agricultural Resources, Ishikawa Agricultural
College, Nonoichi-machi,Ishikawa 921, Japan
Summary
A wheat transformation system was developed by using particle
bombardment and scutellar tissues of immature embryos as its target.
The promoter region preceding a marker gene is one of the most
important factors affecting transformation frequency. In this study,
the 5' upstream sequence of the rice actin 1 gene (Actl)
showed high activity of transient expression in various wheat
cell types including embryogenic call), immature embryos and pollen
embryoids of several wheat accessions. All three callus lines,
including two aneuploid lines, showed high activity of transient
expression of gusA gene. In pollen embryoids, the activity of
transient gusA expression was similar among four wheat cultivars, but
the activity after two days of incubation was slightly higher than
that after five days of incubation in three of cultivars. The
scutellar tissues of both tetraploid and hexaploid wheats provided an
efficient level of the gusA expression. The present findings suggest
that the rice Actl promoter is a useful promoter in the
transformation system of common wheat and its relatives.
Key words: gusA, cultured cells, microprojectile bombardment,
promoter activity, wheat
Introduction
In common wheat (Triticum aestivum L.), particle
bombardment developed by Sanford et al. (1987) is a useful method for
gene delivery to intact cells (Wang et al. 1988). This system has
been widely used as the method for obtaining transgenic wheat plants.
Tranagenic wheat plants have been produced from embryogenic
suspension cells (Vasil et al. 1992) and scutellar tissues of
immature embryos (Weeks et al. 1993; Vasil et al. 1993; Nehra et al.
1994; Becker et al. 1994), but not from other tissues including
pollen embryoids. The pollen embryoid is an attractive target for
particle bombardment because doubled haploid plants can be
regenerated from microspore derived embryos by anther culture
(Henry and De Buyser 1990). The scutellar tissues of immature embryos
are commonly used targets for production of tranagenic plants because
multiple plants can be easily regenerated from the calli induced from
scutellar tissues through immature embryo culture (Scott et al.
1990).
Corresponding author: Shigeo Takurni
-->Next
(go to
KOMUGI Home) (go
to WIS List) (go
to NO.84 Contents)