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Wheat Information Service
December 79: 42-46 (1994)


Two new sources of gametocidal genes from Aegilops longissima and Ae. sharonensis

Hisashi Tsujimoto

Kihara Institute for Biological Research, Yokohama City University, Mutsukawa 3-122-20, Minami-ku, Yokohama 232, Japan


Summary

Two new gametocidal (Gc) genes were identified in the progenies of a cytoplasmic substitution line of common wheat with Ae. longissima cytoplasm and an amphidiploid between T. dicoccum and Ae. sharonensis. C-banding analyses indicated that one gene was located on either chromosome 5S1 of Ae. longissima or an arranged chromosome similar to 5S1, whereas the other was on 4S1 of Ae. sharonensis.


Introduction

Gametocidal (Gc) genes in wheat relatives, which were originally characterized as factors causing gamete abortion, have been revealed to cause chromosome breakage and other abnormal phenomena (Tsujimoto and Tsunewaki 1985, Endo 1988). These genes have been extensively applied for production of chromosome deletion lines in wheat for use in cytological mapping of genes and molecular markers (Tsujimoto and Noda 1990, Werner et al. 1992, Gill et al.1993, Kota et al.1993, Ogihara et al.1994).

So far, several accessions of Aegilops species carrying the C, S or S1 genome have been reported to possess the Gc gene. In the present study two new Gc genes in Ae. longissima and Ae. sharonensis are reported and their characteristics are described.


Materials and methods

The original sources of the present gametocidal genes were the cytoplasmic substitution line of Triticum aestivum cv. Chinese Spring (CS) with the cytoplasm of Aegilops longissima strain TL05 (line C20, BC4F2 generation), and the amphidiploid between T. dicoccum cv. Vernal and Ae. sharonensis strain KU5-1 (F20 generation). These cytoplasmic substitution and amphidiploid lines were originally established by Drs. K. Tsunewaki and M. Sasaki, respectively. These lines were crossed with Chinese Spring, and the seed fertility (seed setting of the first and second florets) and chromosome constitutions of the progenies were observed. The C-banding method of Tsujimoto and Noda (1990) was used for the analysis.

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