Fig 1. Hybridization of the HMW glutenin cDNA to restriction enzyme-digested DNA from amphidiploid and its direct parents. A. Lanes 1, 2, 3, 7 and 9 are in Ae. squarrosa (genomes DDDD) and lanes 4, 5, 6 and 8 are in amphidiploid (DDRR). Lanes 1, 6 and 7 are EcoRI-digestion, lanes 2, 4, 8 and 9 are BamHI-digestion and lanes 3 and 5 are HindIII-digestion. B. Lanes 1, 2, 3 and 7 are in amphidiploid and lanes 4, 5, 6, 8, 9 and 10 are in S. cereale (RRRR). Lanes 1, 4, 7 and 8 are EcoRi-digestion, lanes 2, 5 and 9 are BamHI-digestion, and lanes 3, 6 and 10 are HindIII-digestion.