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Wheat Information
Service
Number 72: 79-82 (1991)
Two
Sec 3 loci of HMW secalin on the long arm of chromosome 1R in rye
(Secale cereale L.)
Y. Yasumuro, N. Nakata and M. Tomita
Faculty of Agriculture, Tottori University, Tottori 680,
Japan
High molecular weight (HMW) secalin genes of endosperm proteins in
rye are useful molecular makers for the long arm of chromosome 1R,
together with a target of bread making quality improvement. The locus
of HMW secalin was mapped and it was designated Sec 3 by
Shewry et al (1984a) and Glu-R1 by Singh and Shepherd
(1984).
We found two different loci of Sec 3 on the 1RL by
F2 analysis using some inbred lines having different HMW
secalin subunits.
Materials and Methods
Four inbred rye lines, IR27, IR48, IR51 and IR94 were chosen from
The Tottori University Collections for making two cross combinations,
IR51 x IR48 and IR27 x IR94. IR5I has three HMW seclain subunits
which were denoted by band Nos.1, 2 and 5 after electrophoresis
fractionation and IR48 has band Nos. 2 and 6 (Fig.
1). IR27 and IR94
have band Nos. 4, 5 and Nos. 2, 4, 5', respectively, where, band No.
5' showed the same mobility but thin staining compared with band No.
5.
F2 seeds were obtained from single F1 plant in
each cross combination. Same weight of flour samples were taken from
single grains in F2 seeds lot and the samples were
fractionated by the SDS-PAGE method by Payne et al (1980) with a
slight modification.
Results of F2 analysis
1. F2 of IR51 (Band Nos. 1, 2, 5) x IR48 (Band Nos. 2, 6):
The bands 1, 5 and 6 segregated in the F2 population
(Fig
1). Results of
the chi2
goodness of fit test
for Mendelian segregation were shown in Table
1. The table
shows that band Nos.1, 5 and 6 were monogenic character and band No.1
vs. No. 6 and No. 5 vs. No. 6 were allelic, but band No.1 vs. No. 5
was nonallelic with considerable linkage. An estimation for
recombination value between the two loci was made by maximum
likelihood method as show in Table
2. Recombination
value between the loci, 1-6 and 5-6, was calculated to be 7.0 + or -
1.0%
2. F2 of IR27 (Band Nos. 4, 5) x
IR94 (Band Nos. 2, 4,
5'): Three kinds of bands, Nos. 2, 5 and 5' segregated in the
F2 population. Results of the chi2
tests indicated that
band No. 2 vs. null and No. 5 vs. No. 5' was allelic
(Table
1). The
recombination value between the two loci was 11.2 + or - 4.1 %.
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