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Number 72: 102 (1991)
Synaptonemal
complex of rye
J. Fujigaki
Tokyo University of Agriculture, Tokyo 156, Japan
Synaptonemal complex (SC) has been studied by thin sections,
reconstructed three dimensional images from many thin sections,
and/or surface spreading of meiocytes in various kind of materials.
Each method has advantages and disadvantages. Well spread SCs in two
dimensions are more favorable to trace whole length of the SC than
the reconstructed ones. Surface spreading methods so far developed
for plant materials are more or less hypotonic bursting technique
with or without enzyme digestion of callose wall of pollen mother
cells. Present report is a preliminary report on the surface
spreading of SCs of rye. The method of Albini and Jones (1984) was
used to make preparations. One of the well spread meiocytes was
photographed by electron microscope (Figure
1.) and each of
the SCs was carefully traced to measure the whole length of the
lateral element. Total length of the seven SCs was 583 micro meter
and relative length of each SC was calculated based upon the total
length. The longest one was 0.19 (actual length, 110 micrometer) and
the shortest was 0. 09 (54 micrometer). This means that the longest
one was twice as long as the shortest one. On the other hand somatic
chromosomes at metaphase in rye range from 7 micrometer to 15
micrometer depending
upon the pretreatment and the difference between the longest and the
shortest is not so large as SCs. Gillies (1985) reported average
length of the lateral element of SCs at zygotene and pachytene in
rye. The longest one in his date was 72.2 micro meter (15.5% length)
and the shortest was 61.8
micrometer (13.3%L) at pachytene of Snoopy. He also obtained similar
result in R119. The
difference between the present result and Gillies' data may be partly
due to the difference of the method. Further study is undergone to
reveal the chromosome architectrure of rye.
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