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Wheat Information Service
Number 71: 16-18 (1990)


Effect of wheat straw extract on the germination and seedlings growth of wheat (cv. Pavon)

S. M. Alam

Atomic Energy Agricultural Research Centre, Tandojam, Pakistan


Abstract

Wheat straw water extract at concentrations of 0.0, 0.2, 0.4 and 0.6% were evaluated to see their effects on the germination and seedling growth of wheat (cv. Pavon) under laboratory condition. With increasing concentrations of residue extract, the germination, shoot and root growth of wheat crop were significnatly decreased. Root growth was affected more than shoot. This may be due to the inhibitory effect of water-soluble substances released from wheat straw.


Introduction

Crop residues on the surface of the soil are very effective in controlling soil erosion by wind and water. However, when crop residues are left on the soil surface, crop yields are occasionally reduced as compared to the incorporation of residues into the soil. Substantial evidence has been accumulated to show that phytotoxic substances are present in most crop plants and may be responsible for reduced crop growth (Borner 1960; Garb 1961). Guenzi and McCalla (1962) showed that the water extracts of a number of crop residues inhibited the germination and growth of sorghum, corn and wheat in a laboratory experiment. LeTourneau et al (1956) found that water extracts from 23 common weed and crop species inhibited germination and growth of wheat seedlings.

Researchers elsewhere have generally shown that allelopathy from wheat residue reduces the subsequent wheat yield (McCalla 1971; McCalla and Haskins 1964; McCalla and Norstadt 1974). They showed that water-soluble substances in crop residues reduced the germination and growth of wheat seedlings and other crops. Allelopathic chemicals from soils, crop residues and weeds are known to reduce the growth of several crops and there are numerous examples of allelopathy among crop plants. Allelopathy is often more evident in disturbed plant communities, such as agricultural ones.

The objective of this investigation is to study the effect of aqueous extract of straw from wheat crop to germination and growth of wheat seedlings (cv. Pavon).


Materials and Methods

i) Preparation of residues: Mature straw of wheat plant was collected from the experimental wheat field at AEARC, farm, Tandojam in April 1989. The samples were first dried in the sun for 12 hr. then transferred to a forced-draft oven for thorough drying at 75 + or - 5C for 24 hr. Dried samples were then ground in a Wiley mill to pass a 1.18
mm2 screen. Ground samples were kept in plastic bags at room temperature.

ii) Laboratory trials: Three levels of wheat straw extracts 0, 0.2, 0.4 and 0.6% (w/v), were prepared for use in petri-dishs germination study with wheat seeds. The extracts were prepared by soaking the dried ground residues in distilled water for 24 hr. at room temperature in 250 ml Erlenmyer flasks. The extracts were then filtered into 100 ml beakers using NO.41 Whatman filter paper.

Wheat seeds were disinfected in 1% sodium hypochlorite solution for 2 minutes and them rinsed with distilled water. Ten healthy wheat seeds were placed in a petri-dish lined with filter paper and 5 ml of extract added from each treatment. The treatment at extract level was replicated three times in a randomized complete block design. All the petri-dishs were kept in an incubator at 28 + or - 2C for 120 hrs. The experiment was terminated after 120 hrs. The number of seed. germinated were counted. Their shoot and root length were also measured. The experiment, was conducted twice and the results were expressed in terms of the averages of the two trials. The data were analyzed to evaluate the treatment effect.

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