| Results A chromatographic survey of IAA-like substances (Fig. 1 & 2) revealed that at the onset of germination as well as on an average the amount of promotors was highest in the tall variety C-306. Intergroup comparison reflected that the single dwarf Sonalika possessed maximum amount of promotors followed by triple dwarf Moti and double dwarf Kalyansona (Table 1). Frequent interconversions within different group of auxin-like substances were quite evident during the 96 hrs of seedling growth. Like the promotors the IAA-like inhibitors were also observed to be highest in C-306. But amongst dwarfs, the Kalyansona which possessed minimum amount of promotors had maximum amount of inhibitors; against this, the Sonalika exhibited lowest level of inhibitors (Table 1). A look at the chromatograms (Fig. 1 & 2) revealed that the inhibitors in C-306 were present between RF 0.8 - 0.9 and/or 0.5 - 0.6 and in Sonalika between 0.5 - 0.6 and 0.0 - 0.1. In Kalyansona and Moti, the picture was not so clear yet the inhibitory substances remained more or less around these RFs. The level of IAA-like promotors reached to the peak in 48 hrs old seedlings while the level of inhibitors was minimum at that time. Gibberellin (GA3) treatment lowered IAA-like inhibitors and enhanced IAA-llke promotors (Table 1). However certain deviations were recorded at varietal level especially in the promotors (Fig. 3). The C-306 and Kalyansona registered a considerable increase in endogenous IAA-like promotors but Sonalika displayed only a marginal increase in the same. On the contrary, the triple dwarf Moti showed a sharp decline in promotors level due to GA3 treatment. In contravention to promotors, all the four varieties exhibited reduction of various magnitude in IAA-like inhibitors as a result of GA3 application. |
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