| Indentification of the chromosomal translocations in
wheat T.M. SHNAIDER & O.J. PRIILINN Department of Plant Genetics, Institute of Experimental Biology of the Academy of Sciences of the Estonian S.S.R., Tallinn, U.S.S.R. Common wheat varieties are often differentiated by one or more chromosomal translocations. A number of chromosomal interchanges has been observed between many hexaploid wheat varieties by various workers (BAKER & MCINTOSH 1966; RILEY et. al.1967; METTIN & KLEIN, 1973; BAIER et. al. 1974; BANNIER 1978; VEGA & LACADENA 1982). Some of these interchanges have been located on specific chromosomes by means of crosses to complete series of 21 monosomic lines of the hexaploid wheat variety Chinese Spring. This paper presents analysis of meiosis in F1 hybrids derived from crosses between the spring wheat variety Norrona and mutant T-13, on one hand, and the monosomic lines of variety Chinese Spring, on the other hand. Materials and Methods Mutant T-13 of the spring wheat variety Norrona was induced by treatment of N-methyl-N-nitrosourea. It has more dense spike, higher productivity and moderate field resistance to leaf rust. Chromosome pairing in pollen mother cells (PMC) was analyzed at the first meiotic metaphase (MI) in F1 hybrids between monosomic plants of each of 21 Chinese Spring (CS) monosomic lines as female parent with mutant T-13 (M8 generation) and variety Norrona (male parents). The somatic chromosome counts of the CS monosomics and the monosomics of F1 crosses were determined using Feulgen method. Spikes of the parents and hybrid F1 plants were fixed in Newcomer's fluid. PMC were observed on acetocarmine smear preparations. Every PMC was scored for the presence of multivalents, and where multivalents occurred, the number of chromosomes involved and their configurations were recorded. |
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