| I. Research Notes Gene flow between the F1 amphidiploid (Aegilops sharonensis x Triticum monococcum) and Triticum turgidum dicoccoides - Further evidence for Aegilops sharonensis as the putative donor of the B genome of wheat U. KUSHNIR and G.M. HALLORAN school of Agriculture and Forestry, University of Melbourne Parkville, Victoria, 3052, Australia Evidence from studies of cytoplasmic compatibility, chromosome pairing, plant morphology and karyomophology (KUSHNIR & HALLORAN 1982a) and from quantitative studies of developmental characters (KUSHNIR & HALLORAN 1982b) have recently been advanced for Ae. sharonensis as the putative donor of the B genome of wheat. The production of a fertile F2 plant from the hybrid T. turgidum dicoccoides x F1 amphidiploid (Ae. sharonensis x T. monococcum) reported in this paper is further support for Ae. sharonensis as the B genome donor of wheat. Material and Methods The amphidiploid used for this study was produced from the hybrid between a line of Ae. sharonensis line from an undisturbed habitat on the coastal plain in Israel and T. monococoum from Turkey. The amphidiploid was produced by the application of 0.05% colchicine solution to the hybrid seedlings. The line of Triticum turgidum dicoccoides used in this study was collected from the Upper Galilee of Israel. The hybrid between T. tuigidum dicoccoides and the F1 amphidiploid Ae. sharonensis x T. monococcum was planted in 15 cm diameter pots containing potting mix (1 part washed sand; 1 part perlite; 1 part Derrimut red brown loam by volume). It was grown in a glasshouse maintained 15-20C in natural daylight over the winter period at Brumley Plant Sciences Research Centre, Mt. Derrimut, Victoria. The somatic chromosome number of the F1 hybrid was determined from root tips of the germinating seed, which had been placed in colchicine (10-3m) for 4 h and stained in 1% acetocarmine for 48 h. For the meiotic studies spikes were collected and fixed in Carnoy's solution for 48 h, stored in 70 percent alcohol and stained in 1 percent acetocarmine. Pollen fertility was determined by dissecting mature anthers soaked in 2% acetocarmine. Grains were considered normal when they were rounded and deeply stained, and a sample of 1000 grains was examined. Seed fertility was determined by examination of two lower florets in the spikelet. A floret was considered fertile if it had a well-developed kernel. In the semi-sterile hybrid plant, seed set was determined by examination of all available spikes. In the more fertile F2 plant a sample of 3000 florets (150 spikelets) was examined. |
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