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Results and Discussion

The differential response between T. turgidum (AABB) and T. timopheevi (AAGG) was shown by the proportion of abortive seed production in the crosses to (squarrosa)AABB+1D lines as seen in Fig. 1. Fig. 1-a shows the crossed seeds obtained from one spike of (squarrosa) T. turgidum+1D line fertilized by the pollen of T. turgidum, in which five normal seeds (in left) had the genetic constitution of (squarrosa)AABB+1D, and were capable to germinate. The other thirty-one abortive seeds (in right) were zygotic lethal, and were presumed to have the genetic constitution of (squarrosa)AABB. In these abortive seeds, the seed coat that originated from tissue of female parent developed, while the embryo and the endosperm did not differenciate.

Fig. 1-b shows the crossed seeds which were obtained from one spike of the same plants as Fig. 1-a fertilized with pollen of T. timopheevi. Majority of female gamete of this (squarrosa) AABB+1D plant must not have 1D chromosome, because the transmission rate of the additional 1D chromosome through female gamete was 24.8% on average. In spite of this low transmission rate of 1D chromosome through female gametes, all zygotes developed to normal seeds, and were able to germinate. This result and the observation of OHTSUKA (1980) suggest that genetic factor(s) of G genome for the genome compatibility with the cytoplasm of Ae. squarrosa is equivalent to that of 1D chromosome.

The differential response of AB and AG genomes to Ae. squarrosa cytoplasm was also observed when (squarrosa)T. dicoccoides+1D-1A and (squarrosa)T. durum+1D were used as female parents in the crosses. The ratio of normal to abortive seeds on female plants of (squarrosa) T. durum+1D was similar to that on (squarrosa) T. turgdium+1D. However, this ratio on (squarrosa) T. dicoccoides+1D-1A line was different from that on either of 1D monosomic-addition lines as shown in Table 1. This might be due to the difference in their transmission rates of 1D chromosome through female gametes.

The results from the crosses of eleven species or strains of tetraploid wheat as pollen parents to the monosomic-addition lines and the monosomic-substitution line having Ae. squarrosa cytoplasm were showed in Table 1. Five species of Emmer wheat and two varieties of Palaestaine-T. dicoccoides were classified as AB type, because many abortive seeds were produced. Percentage of abortive seeds in the crossed seeds were 61.7%-86.0% and 38.1%-61.9% When (squarrosa)AABB+1D and (squarrosa)AABB+1D-1A line were used as female parent, respectively. These figures reflected the frequency of the female gametes without 1D chromosome.

Three strains of Trans-Caucasian-Timopheevi wheat, and two species of Trans-Caucasian-endemic species, T. palaeocolchicum MEN. (=T. georgicum DEK.) and T. persicum VAV. (=T. carthlicum NEVSKI) showed the AG type response, because abortive seeds were not or almost not produced in the crosses. However, T. palaeoclchicum and T. persicum have been classified as Emmer group with AABB genome.



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