| Results and discussion The present method of gel preparation, electrophoresis, fixing and staining gave good banding pattern of water soluble proteins. Since protein banding pattern in embryos will be less affected by environmental conditions embryos are preferred over the whole seed. In general, more as well as denser bands are present on the upper half of the gel. Up to a maximum of 22 bands could be noticed in chinese spring. Fast moving bands are less denser as compared to slow moving bands. More positively charged proteins are towards the top. Lichtis Fruher has extra bands than Roter Lowe. Pakistan II and III showed different banding pattern over Pakistan I (Fig. 1 a and b). This method has many advantages: 1. It takes less time in preparing the protein solution which is to be loaded on the polyacrylamide gel. 2. Fixing and staining is completed within 2 hours. 3. It serves as quick method in identifying and also in eliminating duplicate cultivars. However, this method has one great disadvantage. Since the homogenate is too little, it is very difficult to measure actual protein content we are putting on the gel. Actually how much protein is present in 100 Cmm. of solution is not known. So, the great quantitative differences in the protein composition can be misinterpreted as qualitative differences as the electropheretic mobility of the protein fraction is influenced by the concentration of the protein. Protein estimation by Lowry method is only possible if sufficient quantity of protein solution is available. If some imporvements are made to check the protein content by micro methods this method will be of much use in future. Acknowledgements I wish to express may appreciation to Prof. Dr. Gerhard ROBBELEN for providing the facilities for this study. I thank Dr. Ove HALL and Dr. Henrik JOHANSSON of the Swedish Seed Association, Svalof, Sweden for going through the manuscript and for their helpful comments. I am also indebted to Alexander von Humboldt Stitfung for the award of fellowship. Literature cited DAVIS, B. J. 1964. Disc Electrophoresis-II. Methods and application to human serum proteins. Annals of the New York Academy of Sciences. Vol. 121:404-427. DAVIS, B. J. and L. ORNSTEIN 1959. "A new high resolution electrophoresis method", delivered at The Society for the Study of Blood, March 24, 1959 at the New York Academy of Medicine. (Received February 10, 1972) |
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