| The two new slow moving bands present in the amphiploid of Ae. caudata
G 593 x Ae. umbellulata G 494, but not in the parents, nor in a mixture
of their proteins, may be examples of hybrid protein bands formed by the
combination of two genomes (IRWIN 1932, SCHWARTZ 1960). However, because
the original hybrid was made tetraploid using colchicine, other explanations
must be entertained. Spontaneous autotetraploids of Rubus showed
new chromatographic patterns, as did colchiploids of the same plant (HASKELL
1968). The extra Aegilops bands may be due to the act of tetraploidy
or to gene mutation by colchicine (FOSTER, Ross & FRANZKE 1955). It is also
possible that the amphiploid seed was at a stage of development different
from that of the two parents; the proteins responsible for these two new
bands may have been already broken down, or may have not yet been formed
in the seed tissues of the diploid parents. These possibilities are being
investigated further. Literature cited BELL, G. D. H. 1950. Investigations of the Triticinae I, Colchicine techniques for chromosome doubling in interspecific and intergeneric hybridisation. J. Agric. Sci. 40: 9-18. BREWER, G., C. F. SING and E. R. SEARS 1969. Studies of isozyme patterns in nullisomic-tetrasomic combinations of hexaploid wheat. Proc. Nat. Acad. Sci. U.S. 64: 1224-1229. DESBOROUGH, S. and S. J. PELOQUIN 1966. Disc electrophoresis of tuber proteins from Solanum species and interspecific hybrids. Phytochemistry 5: 727-733. FOSTER, A. E., J. G. Ross and C. J. FRANZKE 1955. Genetic analysis of F2 populations from crosses involving colchicine induced mutants in Sorghum. Proc. S. Dakota Acad. Sci. 34: 21-22. HALL, O. and B. L. JOHNSON 1963. Electrophoretic analysis of the amphiploid of Stipa viridula x Oryzopsis hymenoides and its parental species. Hereditas 48: 530-535. HASKELL, G. 1968. Biochemical differences between spontaneous and colchicine induced autotetraploids. Heredity 23: 139-141. IRWIN, M. R. 1932. Dissimilarities between the antigenic properties of red blood cells of dove hybrids and parental genera. Proc. Soc. Exp. Biol. & Med. 29: 850-851 JOHNSON, B. L. 1967. Confirmation of the genome donors of Aegilops cylindrica. Nature 216: 859-862. JOHNSON, B. L., D. BARNHART and O. HALL 1967. Analysis of genome and species relationships in the polyploid wheats by protein electrophoresis. Amer. Jour. Bot. 54: 1089-l098. KIHARA, H. 1954. Considerations on the evolution and distribution of Aegilops species based on the analyser method. Cytologia 19: 336-357. SCHWARTZ, D, 1960. Genetic studies on mutant enzymes in maize: synthesis of hybrid enzymes by heterozygotes. Proc. Natl. Acad. Sci. U.S. 46: 1210-1215. WAINES, J. G. 1969. Electrophoretic-systematic studies in Aegilops. Ph.D. thesis, University of California, Riverside, California, pp. 223. (Received February 6, 1971) |
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