(go to NO.28 Contents)



With the methods of fractional precipitation with ethanol and pyridine, ion exchange chromatogram and electrophoresis, the substance was purified and isolated and was found to be composed of only amino acids, as was published in Ann. Meet. Agr. Chem. Soc. Japan5 (1967). By further studies, it has been found that moleculer weight of the toxic substance was estimated to be 9,800, and its isoelectric point was higher than pH 10.0. The details are now in press.

Acknowlegement

The authors wish to express their heartfelt thanks to Professor K. YAMASHITA, Kyoto University, for his kindness of supplying the material of wheat species and his valuable advices throughout the present experiments. The authors wish also to express their thanks to Prof. R. TAKAHASHI, Okayama University, for his generosity of supplying barley species.

Literature

1. LECOURT, R. 1928. Untersuchungen uber die Giftigkeit des Weizenmehls gegen Hefe. Wochenshrift fur Brauerei, XLV Jahrgang. Nr. 11: 123-126, 136-139, 148-152.

2. LECOURT, R. 1928. Untersuchungen uber die Giftigkeit des Weizenmehls gegen Hefe. Wochenshrift fur Brauerei, XLV Jahrgang. Nr. 14: 160-163.

3. FINK, H. and F. WEINFURTNER 1930. Die Methylenblaufarbung von Hefezellen und ihre Beziehung zur Wasserstoffzahl und zum Permeabilitatsproblem. Wochenshrift fur Brauerei; XLVII Jahrgang. Nr. 10: 110-115.

4. TAKAHASHI, R. 1955. The origin and evolution of cultivated barley. Advance in Genetics 7: 227 -266.

5. OKADA, T., H. YOSHIZUMI and Y. TERASHIMA 1967. The biological and chemical studies on toxic substance in wheat against brewing yeast. Abstracts of Papers, 38th Meet. Agr. Chem. Soc. Japan. 26-27.

(Received January 9, 1969)



<-- Back      

(go to NO.28 Contents)