| Amount of amino acids in the chlorophyll mutants of
Einkorn wheat Y. ONO and T. FUJII National Institute of Genetics, Misima, Japan Amino acid content in several chlorophyll mutants of Triticum monococcum flavescens was examined at the seedling stage. Four albina, one chlorina and one virido-albina, which has the ability to recover the chlorophyll content, were used in this examination. Normals were used as control. Free amino acid was extracted from young leaves with 75% ethanol, and several amino acids were separated by two dimensional paperchromatographic method. Seven kinds of free amino acids, namely aspartic acid, glutamic acid, glycine, alanine, glutamine, phenyl alanine and leucine, were detected. Four albina and one virido-albina had all of them, while leucine and alanine were not observed either in normals or chlorina. Albina and virido-albina mutants showed larger amounts of all amino acids than the normals and chlorina. Moreover, non-identified spots a and b were observed in all strains, and the size of spot a was larger in normals than in albina. Kinds and amount of organized amino acids were also determined by the same chromatographic method by hydrolysis of fresh leaves. About 14 kinds of organized amino acids were observed in normals, while only 10 kinds were found in albina. Among these components, cysteine, histidine, proline and methionine etc. were not observed in free amino acids, but in albina strains only traces of these were found. Other organized amino acid components occurred in larger amounts in normals than in albina. Leucine, glutamine, alanine and the non-identified spot (a) contained in free amino acid, were not observed in organized amino acids. Therefore, they may be easily transferable. From these results, the albina strains have a larger amount of free amino acids and a smaller amount of organized amino acid than the normals. |