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| 文献 | ||
|---|---|---|
| 著者 | Zheng K., Zhao J., Lin D., Chen J., Xu J., Zhou H., Teng S., Dong Y. | |
| タイトル | The Rice TCM5 Gene Encoding a Novel Deg protease protein is Essential for Chloroplast Development under High Temperatures. | |
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Abstract: High temperature affects a broad spectrum of cellular components and metabolism in plants. The Deg/HtrA family of ATP-independent serine endopeptidases is present in nearly all organisms. Deg proteases are required for the survival of Escherichia coli at high temperatures. However, it is still unclear whether rice Deg proteases are required for chloroplast development under high temperatures. In this study, we reported the first rice deg mutant TCM5 (thermo-sensitive chlorophyll-deficient mutant 5) that has an albino phenotype, defective chloroplasts and could not survive after the 4-5 leaf seedling stage when grown at high temperature (32 °C). However, when grown at low temperatures (20 °C), TCM5 has a normal phenotype. Map-based cloning showed that TCM5 encoding a chloroplast-targeted Deg protease protein. The TCM5 transcripts were highly expressed in all green tissues and undetectable in other tissues, showing the tissue-specific expression. In TCM5 mutants grown at high temperatures, the transcript levels of certain genes associated with chloroplast development especially PSII-associated genes were severely affected, but recovered to normal levels at low temperatures. These results showed important role of TCM5 for chloroplast development under high temperatures. The TCM5 encodes chloroplast-targeted Deg protease protein which is important for chloroplast development and the maintenance of PSII function and its disruption would lead to a defective chloroplast and affected expression levels of genes associated with chloroplast development and photosynthesis at early rice seedling stage under high temperatures. |
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| 掲載誌 | Rice (N Y) | |
| Country | China | |
| 刊数 | 9(1) | |
| ページ | 13 | |
| 発刊年 | 2016 | |
| PubMed ID | 27000876 | |
| PubMed Central ID | 4801845 | |
| DOI | 10.1186/s12284-016-0086-5 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | PSAA PSBA RBCL RBCS RPOB TCM5 V1 V2 V3 _ | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
