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著者 | Huang W., Yu C., Hu J., Wang L., Dan Z., Zhou W., He C., Zeng Y., Yao G., Qi J., Zhang Z., Zhu R., Chen X., Zhu Y. | |
タイトル | Pentatricopeptide-repeat family protein Rf6 functions with hexokinase 6 to rescue rice cytoplasmic male sterility. | |
Abstract: Cytoplasmic male sterility (CMS) has been extensively used for hybrid seed production in many major crops. Honglian CMS (HL-CMS) is one of the three major types of CMS in rice and has contributed greatly to food security worldwide. The HL-CMS trait is associated with an aberrant chimeric mitochondrial transcript, atp6-orfH79, which causes pollen sterility and can be rescued by two nonallelic restorer-of-fertility (Rf) genes, Rf5 or Rf6. Here, we report the identification of Rf6, which encodes a novel pentatricopeptide repeat (PPR) family protein with a characteristic duplication of PPR motifs 3-5. Rf6 is targeted to mitochondria, where it physically associates with hexokinase 6 (OsHXK6) and promotes the processing of the aberrant CMS-associated transcript atp6-orfH79 at nucleotide 1238, which ensures normal pollen development and restores fertility. The duplicated motif 3 of Rf6 is essential for Rf6-OsHXK6 interactions, processing of the aberrant transcript, and restoration of fertility. Furthermore, reductions in the level of OsHXK6 result in atp6-orfH79 transcript accumulation and male sterility. Together these results reveal a novel mechanism for CMS restoration by which Rf6 functions with OsHXK6 to restore HL-CMS fertility. The present study also provides insight into the function of hexokinase 6 in regulating mitochondrial RNA metabolism and may facilitate further exploitation of heterosis in rice. |
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掲載誌 | Proc. Natl. Acad. Sci. U.S.A. | |
Country | China | |
刊数 | 112(48) | |
ページ | 14984-9 | |
発刊年 | 2015 | |
PubMed ID | 26578814 | |
PubMed Central ID | 4672834 | |
DOI | 10.1073/pnas.1511748112 | |
URL | - | |
関連情報 | ||
遺伝子 | HXK6 _ _ _ | |
INSD | - | |
系統 |
野生イネ コアコレクション |
- |
突然変異系統(遺伝研 Collection) | - | |
種子不稔系統 | - | |
致死変異体系統 | - | |
組織別発生段階 - 突然変異系統(遺伝子) |
- | |
栽培イネ品種(遺伝研 Collection) | - | |
組織別発生段階 | - |