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Contents -  About -  - - - - - - Search - strain - polymorphism - chromosome - locus - MGI Position(cM) - Position(bp) -  Map -  Polymorphism (B6, 129/SVJ) Chr. No.: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 X -  Download -  Update -  NIG Mouse Genome Database Last Update:   Feb 26, 2018 Version: 2.8.18		Locus table   Locus Name D1Mit17 Chromosome 1 MGI Position (cM) Position (cM) 110.4 Ensembl Chromosome 1 Position (bp)(Length)(Chr.)(OLD) 191272042-191272208 (167) (Chr.1) Position (GRCm38) 189571254-189571420 (167) (Chr.1) Contact person Toshihiko Shiroishi Sequence Complete Sequence Polymorphism table   Polymorphism MSM>B10=NZB PCR condition   PCR table MgCl2 (mM) 1.5 Taq polymerase BRL Taq DNA polymerase Thermal cycler PERKIN ELMER PJ 2000 PCR buffer x10 Reacton buffer 500mM KCl 100mM Tris-HCl (pH8.3) 15mM MgCl2 0.1% Gelatin   denature anneal extension cycle extension step (72 c) pcr cycle 1 c 94  55  72  10  5  min 90  90  120  cycle 2 c 94  55  72  10  3  min 90  90  120  cycle 3 c 94  58  72  20  10  min 90  90  120  remarks Electrophoresis image Page Top
National Institute of Genetics