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Locus table
  Locus Name D1Mit15
Chromosome 1
MGI Position (cM) 87.9
Position (cM) 86.3
Ensembl Chromosome 1
Position (bp)(Length)(Chr.)(OLD) 170195349-170195508 (160) (Chr.1)
Position (GRCm38) 168358674-168358833 (160) (Chr.1)
Contact person Toshihiko Shiroishi
Sequence Complete Sequence

Polymorphism table
 
Polymorphism NZB>B10=MSM

PCR condition
 
PCR table
MgCl2 (mM) 4
Taq polymerase BRL Taq DNA polymerase
Thermal cycler PERKIN ELMER PJ 2000
PCR buffer x10 Reacton buffer 500mM KCl 100mM Tris-HCl (pH8.3) 40mM MgCl2 0.1% Gelatin
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  10 
min 90  90  120 
cycle 2 c 94  55  72  10 
min 90  90  120 
cycle 3 c 94  58  72  20  10 
min 90  90  120 
remarks

Electrophoresis image
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National Institute of Genetics