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Locus table
  Locus Name D1Mit4
Chromosome 1
MGI Position (cM) 12.0
Position (cM) 8.7
Ensembl Chromosome 1
Position (bp)(Length)(Chr.)(OLD) 21054395-21054587 (193) (Chr.1)
Position (GRCm38) 21069244-21069436 (193) (Chr.1)
Contact person Toshihiko Shiroishi
Sequence Complete Sequence

Polymorphism table
 
Polymorphism DBA/2=B6>MSM>JF1

PCR condition
 
PCR table
MgCl2 (mM) 1.5
Taq polymerase TOYOBO rTaq DNA polymerase
Thermal cycler PERKIN ELMER GeneAmp PCR System 9600
PCR buffer x10 Reacton buffer 500mM KCl 100mM Tris-HCl (pH8.3) 15mM MgCl2
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  35  10 
min 60  60  60 
cycle 2 c          
min      
cycle 3 c          
min      
remarks

Electrophoresis image
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National Institute of Genetics