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Locus table
  Locus Name D1Mit5
Chromosome 1
MGI Position (cM)
Position (cM) 32.8
Ensembl Chromosome 1
Position (bp)(Length)(Chr.)(OLD) 63749807-63749956 (150) (Chr.1), 58201917-58202092 (176) (Chr.1)
Position (GRCm38) 63815531-63815680 (150) (Chr.1)
Contact person Toshihiko Shiroishi
Sequence Complete Sequence

Polymorphism table
 
Polymorphism MSM>NZB>B10

PCR condition
 
PCR table
MgCl2 (mM) 2
Taq polymerase BRL Taq DNA polymerase
Thermal cycler PERKIN ELMER PJ 2000
PCR buffer x10 Reacton buffer 500mM KCl 100mM Tris-HCl (pH8.3) 20mM MgCl2 0.1% Gelatin
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  10 
min 90  90  120 
cycle 2 c 94  55  72  10 
min 90  90  120 
cycle 3 c 94  58  72  20  10 
min 90  90  120 
remarks

Electrophoresis image
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National Institute of Genetics