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Locus table
  Locus Name D1Mit18
Chromosome 1
MGI Position (cM) 29.7
Position (cM) 27.3
Ensembl Chromosome 1
Position (bp)(Length)(Chr.)(OLD) 52363123-52363278 (156) (Chr.1)
Position (GRCm38) 52418578-52418733 (156) (Chr.1)
Contact person Tadao Serikawa
Sequence Complete Sequence

Polymorphism table
 
Polymorphism NC/jic>NC/Nga
Strains / Fragments (bp)
NC/Nga NC/jic
202 203

PCR condition
 
PCR table
MgCl2 (mM) 1.5
Taq polymerase BRL Taq DNA polymerase
Thermal cycler PERKIN ELMER PJ 2000
PCR buffer x10 Reacton buffer 500mM KCl 100mM Tris-HCl (pH8.3) 10-40mM MgCl2 0.1% Gelatin
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  25  10 
min 60  60  30 
cycle 2 c          
min      
cycle 3 c          
min      
remarks Electrophoresis was performed using Nusieve (3:1) 4% gel in 1xTAE

Electrophoresis image
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National Institute of Genetics