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Locus table
  Locus Name D1Mit9
Chromosome 0
MGI Position (cM) 52.0
Position (cM)
Ensembl Chromosome
Position (bp)(Length)(Chr.)(OLD)
Position (GRCm38)
Contact person Tadao Serikawa
Sequence Complete Sequence

Polymorphism table
 
Polymorphism B6=ALY=AEJ>MSM

PCR condition
 
PCR table
MgCl2 (mM) 1.5
Taq polymerase BRL Taq DNA polymerase
Thermal cycler PERKIN ELMER PJ 2000
PCR buffer x10 Reacton buffer 500mM KCl 100mM Tris-HCl (pH8.3) 10-40mM MgCl2 0.1% Gelatin
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  25  10 
min 60  60  30 
cycle 2 c          
min      
cycle 3 c          
min      
remarks Electrophoresis was performed using Nusieve (3:1) 4% gel in 1xTAE

Electrophoresis image
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National Institute of Genetics