 |
 |
| |
Locus Name |
D1Mit111 |
| Chromosome |
1
|
| MGI Position (cM) |
92.3
|
| Position (cM) |
90.34
|
| Ensembl Chromosome |
1
|
| Position (bp)(Length)(Chr.)(OLD) |
170844217-170844386 (170) (Chr.1)
|
| Position (GRCm38) |
169007542-169007711 (171) (Chr.1)
|
| Contact person |
Yoshiaki KIKKAWA |
| Sequence |
Complete Sequence
|
| |
| Polymorphism |
BLG2>CAST>NC/jic>NC/Nga=NC=A/J>SWR>129>SM=SK/Cam>PGN2>NJL>B6=JF1=MSM>DBA=SWN=CBA |
| Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
CBA
|
DBA
|
NC/jic
|
NC
|
NC/Nga
|
PGN2
|
SK/Cam
|
NJL
|
BLG2
|
SWN
|
MSM
|
JF1
|
CAST
|
| 169 |
182 |
185 |
183 |
187 |
168 |
168 |
190 |
187 |
187 |
174 |
182 |
172 |
220 |
168 |
169 |
169 |
199 |
|
|
| |
| PCR table |
| MgCl2 (mM) |
1.5 |
| Taq polymerase |
AmpliTaq Gold |
| Thermal cycler |
GeneAmp 9700 |
| PCR buffer |
AmpliTaq Gold buffer |
| |
denature |
anneal |
extension |
cycle |
extension step (72 c) |
| pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
| min |
0.5 |
0.5 |
1 |
| cycle 2 |
c |
|
|
|
|
|
| min |
|
|
|
| cycle 3 |
c |
|
|
|
|
|
| min |
|
|
|
| remarks |
|
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