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Locus table
  Locus Name D1Mit1
Chromosome 1
MGI Position (cM) 8.7
Position (cM) 4.4
Ensembl Chromosome 1
Position (bp)(Length)(Chr.)(OLD) 11500716-11500840 (125) (Chr.1)
Position (GRCm38) 11515502-11515626 (125) (Chr.1)
Contact person Shigeharu WAKANA
Sequence Complete Sequence

Polymorphism table
 
Polymorphism MSM>>D2=B6>SEG

PCR condition
 
PCR table
MgCl2 (mM) 2
Taq polymerase BRL Taq DNA polymerase
Thermal cycler PERKIN ELMER PJ 2000
PCR buffer x10 Reacton buffer 500mM KCl 100mM Tris-HCl (pH8.3) 10-40mM MgCl2 0.1% Gelatin
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  25  10 
min 60  60  30 
cycle 2 c          
min      
cycle 3 c          
min      
remarks Electrophoresis was performed using Nusieve (3:1) 4% gel in 1xTAE

Electrophoresis image
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National Institute of Genetics