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Locus table
  Locus Name D1Mit167
Chromosome 5
MGI Position (cM) 6.5
Position (cM) 2.2
Ensembl Chromosome 5
Position (bp)(Length)(Chr.)(OLD) 7391155-7391266 (112) (Chr.1), 18006247-18006364 (118) (Chr.14), 3207990-3208105 (116) (Chr.5)
Position (GRCm38) 7401221-7401332 (118) (Chr.1), 3201984-3202099 (118) (Chr.5), 19213121-19213238 (118) (Chr.14)
Contact person Shigeharu WAKANA
Sequence Complete Sequence

Polymorphism table
 
Polymorphism SEG>MSM>B6=D2=C3H

PCR condition
 
PCR table
MgCl2 (mM) 1
Taq polymerase BRL Taq DNA polymerase
Thermal cycler PERKIN ELMER PJ 2000
PCR buffer x10 Reacton buffer 500mM KCl 100mM Tris-HCl (pH8.3) 10-40mM MgCl2 0.1% Gelatin
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  25  10 
min 60  60  30 
cycle 2 c          
min      
cycle 3 c          
min      
remarks

Electrophoresis image
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National Institute of Genetics