|
PCR table |
MgCl2 (mM) |
1 |
Taq polymerase |
BRL Taq DNA polymerase |
Thermal cycler |
PERKIN ELMER PJ 2000 |
PCR buffer |
x10 Reacton buffer 500mM KCl 100mM Tris-HCl (pH8.3) 10-40mM MgCl2 0.1% Gelatin |
|
denature |
anneal |
extension |
cycle |
extension step (72 c) |
pcr |
cycle 1 |
c |
94 |
55 |
72 |
25 |
10 |
min |
60 |
60 |
30 |
cycle 2 |
c |
|
|
|
|
|
min |
|
|
|
cycle 3 |
c |
|
|
|
|
|
min |
|
|
|
remarks |
|
|