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Locus table
  Locus Name D4Mit175
Chromosome 4
MGI Position (cM) 49.6
Position (cM) 41.74
Ensembl Chromosome 4
Position (bp)(Length)(Chr.)(OLD) 99897429-99897539 (111) (Chr.4)
Position (GRCm38) 100399497-100399607 (111) (Chr.4)
Contact person Yoshiaki KIKKAWA
Sequence Complete Sequence

Polymorphism table
 
Polymorphism JF1>NC/Nga=MSM>PGN2>A/J=129=SWR=NC/jic>SM>B6
Strains / Fragments (bp)
B6 SM SWR 129 A/J NC/Nga NC/jic PGN2 MSM JF1
108 122 124 124 124 131 124 126 131 141

PCR condition
 
PCR table
MgCl2 (mM) 1.5
Taq polymerase AmpliTaq Gold
Thermal cycler GeneAmp 9700
PCR buffer AmpliTaq Gold buffer
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  40   
min 0.5  0.5 
cycle 2 c          
min      
cycle 3 c          
min      
remarks

Electrophoresis image

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National Institute of Genetics