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Locus Name |
D4Mit65 |
Chromosome |
4
|
MGI Position (cM) |
69.8
|
Position (cM) |
64.24
|
Ensembl Chromosome |
4
|
Position (bp)(Length)(Chr.)(OLD) |
141117437-141117557 (121) (Chr.4)
|
Position (GRCm38) |
141845361-141845481 (121) (Chr.4)
|
Contact person |
Yoshiaki KIKKAWA |
Sequence |
Complete Sequence
|
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Polymorphism |
129>B6>MSM=A/J>JF1>PGN2=SM>SWR |
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PCR table |
MgCl2 (mM) |
1.5 |
Taq polymerase |
AmpliTaq Gold |
Thermal cycler |
GeneAmp 9700 |
PCR buffer |
AmpliTaq Gold buffer |
|
denature |
anneal |
extension |
cycle |
extension step (72 c) |
pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
min |
0.5 |
0.5 |
1 |
cycle 2 |
c |
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min |
|
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cycle 3 |
c |
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min |
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remarks |
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