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Locus table
  Locus Name D1Mit149
Chromosome 1
MGI Position (cM) 94.2
Position (cM) 91.45
Ensembl Chromosome 1
Position (bp)(Length)(Chr.)(OLD) 174535904-174536005 (102) (Chr.1), 174535752-174535912 (161) (Chr.1)
Position (GRCm38) 172699229-172699330 (150) (Chr.1)
Contact person Yoshiaki KIKKAWA
Sequence Complete Sequence

Polymorphism table
 
Polymorphism SM=129>A/J>SWR>PGN2>B6=JF1=MSM
Strains / Fragments (bp)
B6 SM SWR 129 A/J PGN2 MSM JF1
100 149 119 149 141 116 100 100

PCR condition
 
PCR table
MgCl2 (mM) 1.5
Taq polymerase AmpliTaq Gold
Thermal cycler GeneAmp 9700
PCR buffer AmpliTaq Gold buffer
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  40   
min 0.5  0.5 
cycle 2 c          
min      
cycle 3 c          
min      
remarks

Electrophoresis image

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National Institute of Genetics