|
|
|
Locus Name |
D19Mit33 |
Chromosome |
19
|
MGI Position (cM) |
53.0
|
Position (cM) |
48.64
|
Ensembl Chromosome |
19
|
Position (bp)(Length)(Chr.)(OLD) |
56248882-56249143 (262) (Chr.19)
|
Position (GRCm38) |
56195632-56195893 (262) (Chr.19)
|
Contact person |
Yoshiaki KIKKAWA |
Sequence |
Complete Sequence
|
|
Polymorphism |
CBA>DBA>SK/Cam>PGN2>BLG2=A/J=B6>SWR=NC/Nga=NC=129>JF1=NC/jic>SM>SWN>MSM>CAST>NJL |
Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
CBA
|
DBA
|
NC/Nga
|
NC/jic
|
NC
|
PGN2
|
SK/Cam
|
NJL
|
BLG2
|
SWN
|
MSM
|
JF1
|
CAST
|
261 |
250 |
259 |
259 |
261 |
317 |
315 |
259 |
257 |
259 |
302 |
312 |
208 |
261 |
247 |
246 |
257 |
243 |
|
|
|
PCR table |
MgCl2 (mM) |
1.5 |
Taq polymerase |
AmpliTaq Gold |
Thermal cycler |
GeneAmp 9700 |
PCR buffer |
AmpliTaq Gold buffer |
|
denature |
anneal |
extension |
cycle |
extension step (72 c) |
pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
min |
0.5 |
0.5 |
1 |
cycle 2 |
c |
|
|
|
|
|
min |
|
|
|
cycle 3 |
c |
|
|
|
|
|
min |
|
|
|
remarks |
|
|
|
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|