|
|
|
Locus Name |
D10Mit254 |
Chromosome |
18
|
MGI Position (cM) |
25.5
|
Position (cM) |
16.81
|
Ensembl Chromosome |
18
|
Position (bp)(Length)(Chr.)(OLD) |
42049910-42050033 (124) (Chr.10), 48650310-48650397 (88) (Chr.18)
|
Position (GRCm38) |
42361495-42361618 (124) (Chr.10), 48524980-48525067 (124) (Chr.18)
|
Contact person |
Yoshiaki KIKKAWA |
Sequence |
Complete Sequence
|
|
Polymorphism |
129>JF1>PGN2=A/J=SWR=SM=B6>MSM |
|
|
|
PCR table |
MgCl2 (mM) |
1.5 |
Taq polymerase |
AmpliTaq Gold |
Thermal cycler |
GeneAmp 9700 |
PCR buffer |
AmpliTaq Gold buffer |
|
denature |
anneal |
extension |
cycle |
extension step (72 c) |
pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
min |
0.5 |
0.5 |
1 |
cycle 2 |
c |
|
|
|
|
|
min |
|
|
|
cycle 3 |
c |
|
|
|
|
|
min |
|
|
|
remarks |
|
|
|
|
|