 |
 |
| |
Locus Name |
D8Mit166 |
| Chromosome |
8
|
| MGI Position (cM) |
56.0
|
| Position (cM) |
57.32
|
| Ensembl Chromosome |
8
|
| Position (bp)(Length)(Chr.)(OLD) |
113809962-113810081 (120) (Chr.8)
|
| Position (GRCm38) |
110923285-110923404 (120) (Chr.8)
|
| Contact person |
Yoshiaki KIKKAWA |
| Sequence |
Complete Sequence
|
| |
| Polymorphism |
DBA=JF1=NC=SM=SWR=CBA>A/J>B6>CAST=PGN2>129>SK/Cam>SWN>NJL=BLG2>MSM |
| Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
CBA
|
DBA
|
NC
|
PGN2
|
SK/Cam
|
NJL
|
BLG2
|
SWN
|
MSM
|
JF1
|
CAST
|
| 118 |
122 |
122 |
115 |
119 |
122 |
122 |
122 |
117 |
110 |
102 |
102 |
106 |
100 |
122 |
117 |
|
|
| |
| PCR table |
| MgCl2 (mM) |
1.5 |
| Taq polymerase |
AmpliTaq Gold |
| Thermal cycler |
GeneAmp 9700 |
| PCR buffer |
AmpliTaq Gold buffer |
| |
denature |
anneal |
extension |
cycle |
extension step (72 c) |
| pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
| min |
0.5 |
0.5 |
1 |
| cycle 2 |
c |
|
|
|
|
|
| min |
|
|
|
| cycle 3 |
c |
|
|
|
|
|
| min |
|
|
|
| remarks |
|
|
|
|
|
