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Locus table
  Locus Name D4Mit225
Chromosome 4
MGI Position (cM) 80.0
Position (cM) 65.35
Ensembl Chromosome 4
Position (bp)(Length)(Chr.)(OLD) 148391903-148392052 (150) (Chr.4), 148391772-148391911 (140) (Chr.4)
Position (GRCm38)
Contact person Yoshiaki KIKKAWA
Sequence Complete Sequence

Polymorphism table
 
Polymorphism MSM>SWN=CAST>BLG2=JF1>PGN2>CBA>A/J=NC>B6=SM>DBA>SK/Cam>NJL>129>SWR
Strains / Fragments (bp)
B6 SM SWR 129 A/J CBA DBA NC PGN2 SK/Cam NJL BLG2 SWN MSM JF1 CAST
154 154 137 139 155 157 153 155 161 147 146 165 169 177 165 169

PCR condition
 
PCR table
MgCl2 (mM) 1.5
Taq polymerase AmpliTaq Gold
Thermal cycler GeneAmp 9700
PCR buffer AmpliTaq Gold buffer
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  40   
min 0.5  0.5 
cycle 2 c          
min      
cycle 3 c          
min      
remarks

Electrophoresis image


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National Institute of Genetics