|
|
|
Locus Name |
D3Mit73 |
Chromosome |
3
|
MGI Position (cM) |
39.7
|
Position (cM) |
30.37
|
Ensembl Chromosome |
3
|
Position (bp)(Length)(Chr.)(OLD) |
85485123-85485268 (146) (Chr.3)
|
Position (GRCm38) |
85399196-85399341 (146) (Chr.3)
|
Contact person |
Yoshiaki KIKKAWA |
Sequence |
Complete Sequence
|
|
Polymorphism |
PGN2>B6=SWR>MSM>JF1>SM>A/J>129 |
Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
PGN2
|
MSM
|
JF1
|
150 |
122 |
150 |
104 |
106 |
152 |
126 |
124 |
|
|
|
PCR table |
MgCl2 (mM) |
1.5 |
Taq polymerase |
AmpliTaq Gold |
Thermal cycler |
GeneAmp 9700 |
PCR buffer |
AmpliTaq Gold buffer |
|
denature |
anneal |
extension |
cycle |
extension step (72 c) |
pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
min |
0.5 |
0.5 |
1 |
cycle 2 |
c |
|
|
|
|
|
min |
|
|
|
cycle 3 |
c |
|
|
|
|
|
min |
|
|
|
remarks |
|
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|