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Locus table
  Locus Name D14Mit8
Chromosome 14
MGI Position (cM) 48.2
Position (cM) 53.05
Ensembl Chromosome 14
Position (bp)(Length)(Chr.)(OLD) 100421498-100421701 (204) (Chr.14)
Position (GRCm38) 101536132-101536335 (204) (Chr.14)
Contact person Yoshiaki KIKKAWA
Sequence Complete Sequence

Polymorphism table
 
Polymorphism 129>A/J=B6=SM=SWR>PGN2>JF1=MSM
Strains / Fragments (bp)
B6 SM SWR 129 A/J PGN2 MSM JF1
207 207 207 211 207 203 196 196

PCR condition
 
PCR table
MgCl2 (mM) 1.5
Taq polymerase AmpliTaq Gold
Thermal cycler GeneAmp 9700
PCR buffer AmpliTaq Gold buffer
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  40   
min 0.5  0.5 
cycle 2 c          
min      
cycle 3 c          
min      
remarks

Electrophoresis image

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National Institute of Genetics