|
|
|
Locus Name |
D13Mit36 |
Chromosome |
0
|
MGI Position (cM) |
53.0
|
Position (cM) |
42.61
|
Ensembl Chromosome |
|
Position (bp)(Length)(Chr.)(OLD) |
|
Position (GRCm38) |
|
Contact person |
Yoshiaki KIKKAWA |
Sequence |
Complete Sequence
|
|
Polymorphism |
BLG2=SK/Cam>SWR>129>A/J>DBA=NC=CBA>PGN2=SM>B6>NJL>CAST=MSM=JF1=SWN |
Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
CBA
|
DBA
|
NC
|
PGN2
|
SK/Cam
|
NJL
|
BLG2
|
SWN
|
MSM
|
JF1
|
CAST
|
141 |
143 |
150 |
146 |
145 |
144 |
144 |
144 |
143 |
155 |
132 |
155 |
128 |
128 |
128 |
128 |
|
|
|
PCR table |
MgCl2 (mM) |
1.5 |
Taq polymerase |
AmpliTaq Gold |
Thermal cycler |
GeneAmp 9700 |
PCR buffer |
AmpliTaq Gold buffer |
|
denature |
anneal |
extension |
cycle |
extension step (72 c) |
pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
min |
0.5 |
0.5 |
1 |
cycle 2 |
c |
|
|
|
|
|
min |
|
|
|
cycle 3 |
c |
|
|
|
|
|
min |
|
|
|
remarks |
|
|
|
|
|