 |
 |
| |
Locus Name |
D2Mit106 |
| Chromosome |
2
|
| MGI Position (cM) |
75.6
|
| Position (cM) |
63.37
|
| Ensembl Chromosome |
2
|
| Position (bp)(Length)(Chr.)(OLD) |
132563529-132563678 (150) (Chr.2)
|
| Position (GRCm38) |
132872088-132872237 (150) (Chr.2)
|
| Contact person |
Yoshiaki KIKKAWA |
| Sequence |
Complete Sequence
|
| |
| Polymorphism |
SWN>BLG2=JF1>MSM>NJL>PGN2>SK/Cam=CBA>129=SM>CAST>B6=DBA=NC=SWR>A/J |
| Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
CBA
|
DBA
|
NC
|
PGN2
|
SK/Cam
|
NJL
|
BLG2
|
SWN
|
MSM
|
JF1
|
CAST
|
| 150 |
153 |
150 |
153 |
149 |
154 |
150 |
150 |
167 |
154 |
202 |
204 |
218 |
203 |
204 |
152 |
|
|
| |
| PCR table |
| MgCl2 (mM) |
1.5 |
| Taq polymerase |
AmpliTaq Gold |
| Thermal cycler |
GeneAmp 9700 |
| PCR buffer |
AmpliTaq Gold buffer |
| |
denature |
anneal |
extension |
cycle |
extension step (72 c) |
| pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
| min |
0.5 |
0.5 |
1 |
| cycle 2 |
c |
|
|
|
|
|
| min |
|
|
|
| cycle 3 |
c |
|
|
|
|
|
| min |
|
|
|
| remarks |
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