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Locus table
  Locus Name D5Mit158
Chromosome 5
MGI Position (cM) 62.0
Position (cM) 49.64
Ensembl Chromosome 5
Position (bp)(Length)(Chr.)(OLD) 115224168-115224480 (313) (Chr.5)
Position (GRCm38) 114963169-114963481 (313) (Chr.5)
Contact person Yoshiaki KIKKAWA
Sequence Complete Sequence

Polymorphism table
 
Polymorphism A/J=SM=SWR>PGN2>129>B6>JF1=MSM
Strains / Fragments (bp)
B6 SM SWR 129 A/J PGN2 MSM JF1
310 331 331 311 331 314 296 296

PCR condition
 
PCR table
MgCl2 (mM) 1.5
Taq polymerase AmpliTaq Gold
Thermal cycler GeneAmp 9700
PCR buffer AmpliTaq Gold buffer
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  40   
min 0.5  0.5 
cycle 2 c          
min      
cycle 3 c          
min      
remarks

Electrophoresis image

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National Institute of Genetics