|
|
|
Locus Name |
D5Mit23 |
Chromosome |
0
|
MGI Position (cM) |
54.0
|
Position (cM) |
38.39
|
Ensembl Chromosome |
|
Position (bp)(Length)(Chr.)(OLD) |
|
Position (GRCm38) |
|
Contact person |
Yoshiaki KIKKAWA |
Sequence |
Complete Sequence
|
|
Polymorphism |
PGN2>DBA>129>SM>NC=A/J=SWR=B6>CAST>CBA>SK/Cam>BLG2>JF1=NJL=SWN>MSM |
Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
CBA
|
DBA
|
NC
|
PGN2
|
SK/Cam
|
NJL
|
BLG2
|
SWN
|
MSM
|
JF1
|
CAST
|
195 |
197 |
195 |
198 |
195 |
179 |
200 |
195 |
202 |
178 |
167 |
168 |
167 |
166 |
167 |
184 |
|
|
|
PCR table |
MgCl2 (mM) |
1.5 |
Taq polymerase |
AmpliTaq Gold |
Thermal cycler |
GeneAmp 9700 |
PCR buffer |
AmpliTaq Gold buffer |
|
denature |
anneal |
extension |
cycle |
extension step (72 c) |
pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
min |
0.5 |
0.5 |
1 |
cycle 2 |
c |
|
|
|
|
|
min |
|
|
|
cycle 3 |
c |
|
|
|
|
|
min |
|
|
|
remarks |
|
|
|
|
|