|
|
|
Locus Name |
D8Mit172 |
Chromosome |
8
|
MGI Position (cM) |
8.0
|
Position (cM) |
7.67
|
Ensembl Chromosome |
8
|
Position (bp)(Length)(Chr.)(OLD) |
17803980-17804233 (254) (Chr.8)
|
Position (GRCm38) |
17804003-17804256 (254) (Chr.8)
|
Contact person |
Yoshiaki KIKKAWA |
Sequence |
Complete Sequence
|
|
Polymorphism |
PGN2>CAST>B6>SK/Cam=A/J=129=SM>SWR>NC=DBA=CBA>SWN>MSM>JF1>BLG2>NJL |
Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
CBA
|
DBA
|
NC
|
PGN2
|
SK/Cam
|
NJL
|
BLG2
|
SWN
|
MSM
|
JF1
|
CAST
|
253 |
251 |
250 |
251 |
251 |
249 |
249 |
249 |
270 |
251 |
212 |
219 |
227 |
222 |
220 |
269 |
|
|
|
PCR table |
MgCl2 (mM) |
1.5 |
Taq polymerase |
AmpliTaq Gold |
Thermal cycler |
GeneAmp 9700 |
PCR buffer |
AmpliTaq Gold buffer |
|
denature |
anneal |
extension |
cycle |
extension step (72 c) |
pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
min |
0.5 |
0.5 |
1 |
cycle 2 |
c |
|
|
|
|
|
min |
|
|
|
cycle 3 |
c |
|
|
|
|
|
min |
|
|
|
remarks |
|
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