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Locus table
  Locus Name D4Mit253
Chromosome 4
MGI Position (cM) 81.5
Position (cM) 70.33
Ensembl Chromosome 4
Position (bp)(Length)(Chr.)(OLD) 151849637-151849783 (147) (Chr.4), 151849527-151849652 (126) (Chr.4)
Position (GRCm38) 153005837-153005983 (147) (Chr.4)
Contact person Yoshiaki KIKKAWA
Sequence Complete Sequence

Polymorphism table
 
Polymorphism PGN2>A/J=B6=SWR>MSM=SM=129>JF1
Strains / Fragments (bp)
B6 SM SWR 129 A/J PGN2 MSM JF1
148 144 148 144 148 152 144 142

PCR condition
 
PCR table
MgCl2 (mM) 1.5
Taq polymerase AmpliTaq Gold
Thermal cycler GeneAmp 9700
PCR buffer AmpliTaq Gold buffer
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  40   
min 0.5  0.5 
cycle 2 c          
min      
cycle 3 c          
min      
remarks

Electrophoresis image

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National Institute of Genetics