 |
 |
| |
Locus Name |
D4Mit27 |
| Chromosome |
4
|
| MGI Position (cM) |
42.5
|
| Position (cM) |
31.32
|
| Ensembl Chromosome |
4
|
| Position (bp)(Length)(Chr.)(OLD) |
88535063-88535210 (148) (Chr.4)
|
| Position (GRCm38) |
89063832-89063979 (150) (Chr.4)
|
| Contact person |
Yoshiaki KIKKAWA |
| Sequence |
Complete Sequence
|
| |
| Polymorphism |
CAST>NC>DBA>A/J>B6>129>PGN2>CBA=MSM=SK/Cam=JF1=NJL=SWN>SWR>SM>BLG2 |
| Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
CBA
|
DBA
|
NC
|
PGN2
|
SK/Cam
|
NJL
|
BLG2
|
SWN
|
MSM
|
JF1
|
CAST
|
| 151 |
115 |
116 |
138 |
153 |
117 |
154 |
158 |
119 |
117 |
117 |
106 |
117 |
117 |
117 |
166 |
|
|
| |
| PCR table |
| MgCl2 (mM) |
1.5 |
| Taq polymerase |
AmpliTaq Gold |
| Thermal cycler |
GeneAmp 9700 |
| PCR buffer |
AmpliTaq Gold buffer |
| |
denature |
anneal |
extension |
cycle |
extension step (72 c) |
| pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
| min |
0.5 |
0.5 |
1 |
| cycle 2 |
c |
|
|
|
|
|
| min |
|
|
|
| cycle 3 |
c |
|
|
|
|
|
| min |
|
|
|
| remarks |
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