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Locus table
  Locus Name D8Mit205
Chromosome 8
MGI Position (cM) 30.0
Position (cM) 32.73
Ensembl Chromosome 8
Position (bp)(Length)(Chr.)(OLD) 52336179-52336319 (141) (Chr.8)
Position (GRCm38) 50837362-50837502 (141) (Chr.8)
Contact person Yoshiaki KIKKAWA
Sequence Complete Sequence

Polymorphism table
 
Polymorphism SM>B6=MSM>JF1>A/J=SWR>PGN2>129
Strains / Fragments (bp)
B6 SM SWR 129 A/J PGN2 MSM JF1
141 159 135 127 135 129 141 136

PCR condition
 
PCR table
MgCl2 (mM) 1.5
Taq polymerase AmpliTaq Gold
Thermal cycler GeneAmp 9700
PCR buffer AmpliTaq Gold buffer
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  40   
min 0.5  0.5 
cycle 2 c          
min      
cycle 3 c          
min      
remarks

Electrophoresis image

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National Institute of Genetics