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![](/mouse/mmdbj/images/titleMicrosatelliteEn.gif;jsessionid=DED27AE8EE7BCD16F5EBEA6378300F2D) |
|
Locus Name |
D18Mit142 |
Chromosome |
18
|
MGI Position (cM) |
47.0
|
Position (cM) |
32.54
|
Ensembl Chromosome |
18
|
Position (bp)(Length)(Chr.)(OLD) |
75514318-75514437 (120) (Chr.18)
|
Position (GRCm38) |
75388953-75389072 (120) (Chr.18)
|
Contact person |
Yoshiaki KIKKAWA |
Sequence |
Complete Sequence
|
|
Polymorphism |
CAST>NJL>JF1=MSM=SK/Cam>DBA=129=CBA>PGN2>SWN>SM>SWR=NC=B6=A/J>BLG2 |
Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
CBA
|
DBA
|
NC
|
PGN2
|
SK/Cam
|
NJL
|
BLG2
|
SWN
|
MSM
|
JF1
|
CAST
|
118 |
120 |
118 |
130 |
118 |
130 |
130 |
118 |
129 |
132 |
135 |
0 |
127 |
132 |
132 |
150 |
|
|
|
PCR table |
MgCl2 (mM) |
1.5 |
Taq polymerase |
AmpliTaq Gold |
Thermal cycler |
GeneAmp 9700 |
PCR buffer |
AmpliTaq Gold buffer |
|
denature |
anneal |
extension |
cycle |
extension step (72 c) |
pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
min |
0.5 |
0.5 |
1 |
cycle 2 |
c |
|
|
|
|
|
min |
|
|
|
cycle 3 |
c |
|
|
|
|
|
min |
|
|
|
remarks |
|
|
|
|
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