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Locus table
  Locus Name D4Mit254
Chromosome 4
MGI Position (cM) 82.5
Position (cM) 74.63
Ensembl Chromosome 4
Position (bp)(Length)(Chr.)(OLD) 152563665-152563799 (135) (Chr.4)
Position (GRCm38) 153719865-153719999 (135) (Chr.4)
Contact person Yoshiaki KIKKAWA
Sequence Complete Sequence

Polymorphism table
 
Polymorphism SWN>SM>CAST=CBA=A/J=SWR=B6>NC=DBA>PGN2>MSM=JF1>SK/Cam>NJL>BLG2>129
Strains / Fragments (bp)
B6 SM SWR 129 A/J CBA DBA NC PGN2 SK/Cam NJL BLG2 SWN MSM JF1 CAST
134 136 134 111 134 134 133 133 130 117 116 114 137 120 120 134

PCR condition
 
PCR table
MgCl2 (mM) 1.5
Taq polymerase AmpliTaq Gold
Thermal cycler GeneAmp 9700
PCR buffer AmpliTaq Gold buffer
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  40   
min 0.5  0.5 
cycle 2 c          
min      
cycle 3 c          
min      
remarks

Electrophoresis image


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National Institute of Genetics