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|
Locus Name |
D10Mit20 |
Chromosome |
10
|
MGI Position (cM) |
35.0
|
Position (cM) |
23.49
|
Ensembl Chromosome |
10
|
Position (bp)(Length)(Chr.)(OLD) |
66407765-66407990 (226) (Chr.10)
|
Position (GRCm38) |
67012408-67012633 (234) (Chr.10)
|
Contact person |
Yoshiaki KIKKAWA |
Sequence |
Complete Sequence
|
|
Polymorphism |
SWR=PGN2>A/J=129>B6>MSM>JF1>SM |
Strains / Fragments (bp) |
B6
|
SM
|
SWR
|
129
|
A/J
|
PGN2
|
MSM
|
JF1
|
228 |
172 |
233 |
229 |
229 |
233 |
209 |
205 |
|
|
|
PCR table |
MgCl2 (mM) |
1.5 |
Taq polymerase |
AmpliTaq Gold |
Thermal cycler |
GeneAmp 9700 |
PCR buffer |
AmpliTaq Gold buffer |
|
denature |
anneal |
extension |
cycle |
extension step (72 c) |
pcr |
cycle 1 |
c |
94 |
55 |
72 |
40 |
|
min |
0.5 |
0.5 |
1 |
cycle 2 |
c |
|
|
|
|
|
min |
|
|
|
cycle 3 |
c |
|
|
|
|
|
min |
|
|
|
remarks |
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