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Locus table
  Locus Name D12Mit84
Chromosome 12
MGI Position (cM) 11.0
Position (cM) 8.26
Ensembl Chromosome 12
Position (bp)(Length)(Chr.)(OLD) 28156911-28157078 (168) (Chr.12)
Position (GRCm38) 27570643-27570810 (172) (Chr.12)
Contact person Yoshiaki KIKKAWA
Sequence Complete Sequence

Polymorphism table
 
Polymorphism MSM>JF1>B6=SM>A/J>PGN2>SWR=129
Strains / Fragments (bp)
B6 SM SWR 129 A/J PGN2 MSM JF1
172 172 159 159 163 161 187 178

PCR condition
 
PCR table
MgCl2 (mM) 1.5
Taq polymerase AmpliTaq Gold
Thermal cycler GeneAmp 9700
PCR buffer AmpliTaq Gold buffer
  denature anneal extension cycle extension step (72 c)
pcr cycle 1 c 94  55  72  40   
min 0.5  0.5 
cycle 2 c          
min      
cycle 3 c          
min      
remarks

Electrophoresis image

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National Institute of Genetics